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KMID : 0613820080180040454
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Journal of Life Science
2008 Volume.18 No. 4 p.454 ~ p.460
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Morphological, Cytological and Molecular Evidence for Intersubgeneric F1 Hybrid between Glycine max x G. tomentella
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Choi In-Soo
Kim Yung-Chul
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Abstract
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This study was carried out to demonstrate morphological, cytological and molecular evidence for intersubgeneric hybrid between Glycine tomentella and G. max cv. ‘Baemkong’. Morphological features of F1 plant for pistil and stamen, flower color and growth habit showed intermediate type between G. tomentella and G. max cv. ‘Baemkong’. Chromosome number of plant was 2n=39, which explained the evidence of F1 hybrid between G. tomentella (2n=38) and G. max cv. ‘Baemkong’ (2n=40). Polyacrylamide isoelectric focusing pattern for esterase and peroxidase also showed that the plant was true hybrid between G. tomentella and G. max cv. ‘Baemkong’. From RAPD analysis, we identified that 62 primers showing bands in hybrid had both bands from G. tomentella and G. max cv. ‘Baemkong’, which suggested that this was true F1 hybrid. Based on our results from morphological, cytological and molecular analyses, we suggest that the F1 plant was true intersubgeneric hybrid between G. tomentella and G. max cv. ‘Baemkong’. Our results still remain us further study to recover fertility of F1 hybrids. The occurrence of maternal and/or paternal inheritance in F1 hybrid from intersubgeneric cross between G. tomentella and G. max cv. ‘Baemkong’ need to be explained.
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KEYWORD
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G. max, G. tomentellaF1 , hybrid, morphological traits, chromosome number, polyacrylamide isoelectric focusing, RAPD
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